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Crowded city buses tend to have poor ventilation, especially in winter. As a result, carbon dioxide builds up in the interior space, causing temporary health problems such as lethargy and hypoxia in passengers. Poor ventilation also promotes the transmission of bacterial and viral infections. Therefore, bus passengers, and especially vulnerable groups such as new and expectant mothers, the elderly and young children, would benefit greatly from this ecological air-exchange system using the alga chlorella, which absorbs carbon dioxide and releases oxygen.